rpoB Gene Sequencing for Identification of Corynebacterium Species
نویسندگان
چکیده
منابع مشابه
rpoB gene sequencing for identification of Corynebacterium species.
The genus Corynebacterium is a heterogeneous group of species comprising human and animal pathogens and environmental bacteria. It is defined on the basis of several phenotypic characters and the results of DNA-DNA relatedness and, more recently, 16S rRNA gene sequencing. However, the 16S rRNA gene is not polymorphic enough to ensure reliable phylogenetic studies and needs to be completely sequ...
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3 Rina de Zwaan, Jakko van Ingen, and Dick van Soolingen 4 5 National Mycobacteria Reference Laboratory, National Institute for Public Health and the Environment 6 (RIVM), Bilthoven, the Netherlands 7 Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands 8 9 10 #Corresponding author: Prof. Dr. D. van Soolingen, [email protected] 11 12 13 Runni...
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Acinetobacter species are defined on the basis of several phenotypic characters, results of DNA-DNA homology, and more recently, similarities or dissimilarities in 16S rRNA gene sequences. However, the 16S rRNA gene is not polymorphic enough to clearly distinguish all Acinetobacter species. We used an RNA polymerase beta-subunit gene (rpoB)-based identification scheme for the delineation of spe...
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The complete sequence of rpoB, the gene encoding the beta subunit of RNA polymerase was determined for Staphylococcus saccharolyticus, Staphylococcus lugdunensis, S taphylococcus caprae, and Staphylococcus intermedius and partial sequences were obtained for an additional 27 Staphylococcus species. The complete rpoB sequences varied in length from 3,452 to 3,845 bp and had a 36.8 to 39.2% GC con...
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In the Netherlands, clinical isolation of nontuberculous mycobacteria (NTM) has increased over the past decade. Proper identification of isolates is important, as NTM species differ strongly in clinical relevance. Most of the currently applied identification methods cannot distinguish between all different Mycobacterium species and complexes within species. rpoB gene sequencing exhibits a promi...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.9.3925-3931.2004